How many PCR reactions will I be able to run with my probe or primer?

The number of reactions you can run with a given amount of probe or primer is dependent on a few factors, such as input concentration of the primer or probe and the final yield of the synthesis product, which can be variable. If you plan to use your primer for PCR, 1 nmol of product will provide for approximately 100 PCR reactions (when the primer is used at a 200 to 250 nmol concentration in a 50 µL reaction). As for probes, 1 nmol of product is usually enough to run 100-300 reactions but again, this depends on the concentration of probe you are using in your reaction.

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