What is the difference between the FAM-BHQ ValuProbe priced at $95 and the $150 FAM-BHQ probe? |
The delivery amount for the Valuprobe is 10 nmole, while the standard $150 probe has a minimum delivery of 10 nmole but averages closer to 20 nmole. The other difference is the level of purification. The Valuprobe is purified using Reverse Phase HPLC so that residual fluorescent contamination and other synthesis impurities are removed. The $150 probe is purified by dual HPLC (anion exchange HPLC followed by reverse phase HPLC) which ensures the absolute highest quality in the manufacture of BHQ-quenched dual label probes. The level of purification is dependent on the researchers preference for purification stringency. It’s recommended to use dual HPLC purified probes for multiplex reactions. For more detailed information on the probe quality comparisons, please contact techsupport@biosearchtech.com










