How is the Tm calculated in Biosearch’s Real Time Design Software?

Our design software, RealTimeDesign (RTD) calculates TM’s using the unified nearest neighbor parameters established by Santa Lucia. Tm’s calculated on RTD may have discrepancies when compared to other design software's provided by ABI, Exiqon, and IDT. RealTimeDesign’s Tms are in very good agreement with the software programs provided by John SantaLucia and Michael Zuker. These programs are available for free over the internet:

SantaLucia: http://ozone3.chem.wayne.edu
Zuker: http://www.bioinfo.rpi.edu/applications/hybrid/

FAQs