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BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
qPCR Assay Design & Optimization |
Do I need to calibrate my instrument to detect your Pulsar® 650 dye?
Why do I get different Ct values for the same probe sequence when labeled with a different dye?
How are fluorescent labels on dual-labeled probes accounted for when measuring the absorbance?
How do freeze thaw cycles affect oligonucleotides?
How do I calibrate my instrument for the CAL Fluor Dyes?
How do I determine the amount of diluent to add to my probe to prepare a stock concentration?
How do I determine which design mode to use in RealTimeDesign?
How do I enter in a SNP, MNP or InDel sequence into RealTimeDesign?
How do I find the primers and probe sequences after I design them?
How do I know what fluorophore to pick for my Dual-labeled probe?
How do I order primers and probes after I design them?
How do I quantitate oligonucleotides by spectrophotometer?
How many PCR reactions will I be able to run with my probe or primer?
I am having a problem with background drift with my Dual-labeled probe, what could be happening?
I am not getting any signal generation with my Dual-labeled probe. What could be going on here?
I am performing Real Time PCR and for my negative controls I am getting products. What could be going on here?
I am using a BHQ labeled probe, how do I adjust my thermal cycler’s settings to account for the quencher?
If I have my own primer designs, can RealTimeDesign (RTD) design only the probe for me?
If I left my oligos sitting on the lab bench over the weekend, are they still ok to use?
If I order a dual-labeled probe with an internal BHQ modification, what will be at the 3’ end of the probe?
Is there any way of designating a specific region on my sequence where RealTimeDesign can design my primers, probes and assays?
What are Tandem Repeats and Mask Tandems in RealTimeDesign?
What does the Overall Rank score in RealTimeDesign represent?
What is the difference between the FAM-BHQ ValuProbe priced at $95 and the $150 FAM-BHQ probe?
What is the Failure Count Data in RealTimeDesign?
What is your recommended method for reconstituting and storing oligos?
When I received my Molecular Beacon probe I also received a complement, what is this for?
Where can I find information that explains the differences between each of the probes you offer?
Why is my BHQ-Pulsar probe not detected in a singleplex reaction on the Lightcycler?
I am a beginner at Real Time PCR, do you have any information to help me design my Dual-labeled assay?
Does Biosearch have software available for designing probes and primers?
What are the differences between the parameter settings in your primer and probe design software (RealTimeDesign)?
How is the Tm calculated in Biosearch’s Real Time Design Software?
Is there a formula for calculating the efficiencies of singleplex PCR reactions?
Why do I get different Ct values for the same probe sequence when labeled with a different dye?
How are fluorescent labels on dual-labeled probes accounted for when measuring the absorbance?
How do freeze thaw cycles affect oligonucleotides?
How do I calibrate my instrument for the CAL Fluor Dyes?
How do I determine the amount of diluent to add to my probe to prepare a stock concentration?
How do I determine which design mode to use in RealTimeDesign?
How do I enter in a SNP, MNP or InDel sequence into RealTimeDesign?
How do I find the primers and probe sequences after I design them?
How do I know what fluorophore to pick for my Dual-labeled probe?
How do I order primers and probes after I design them?
How do I quantitate oligonucleotides by spectrophotometer?
How many PCR reactions will I be able to run with my probe or primer?
I am having a problem with background drift with my Dual-labeled probe, what could be happening?
I am not getting any signal generation with my Dual-labeled probe. What could be going on here?
I am performing Real Time PCR and for my negative controls I am getting products. What could be going on here?
I am using a BHQ labeled probe, how do I adjust my thermal cycler’s settings to account for the quencher?
If I have my own primer designs, can RealTimeDesign (RTD) design only the probe for me?
If I left my oligos sitting on the lab bench over the weekend, are they still ok to use?
If I order a dual-labeled probe with an internal BHQ modification, what will be at the 3’ end of the probe?
Is there any way of designating a specific region on my sequence where RealTimeDesign can design my primers, probes and assays?
What are Tandem Repeats and Mask Tandems in RealTimeDesign?
What does the Overall Rank score in RealTimeDesign represent?
What is the difference between the FAM-BHQ ValuProbe priced at $95 and the $150 FAM-BHQ probe?
What is the Failure Count Data in RealTimeDesign?
What is your recommended method for reconstituting and storing oligos?
When I received my Molecular Beacon probe I also received a complement, what is this for?
Where can I find information that explains the differences between each of the probes you offer?
Why is my BHQ-Pulsar probe not detected in a singleplex reaction on the Lightcycler?
I am a beginner at Real Time PCR, do you have any information to help me design my Dual-labeled assay?
Does Biosearch have software available for designing probes and primers?
What are the differences between the parameter settings in your primer and probe design software (RealTimeDesign)?
How is the Tm calculated in Biosearch’s Real Time Design Software?
Is there a formula for calculating the efficiencies of singleplex PCR reactions?
