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BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
Applications |
Stellaris RNA FISH
Stellaris FISH Probes are used for single molecule RNA detection by fluorescence in situ hybridization (FISH). The Stellaris FISH method is a much improved and simplified FISH technology that requires no probe preparation and can be performed in four easy steps.
RealTimeDesign Software
RealTimeDesign™ is our web-based qPCR assay design software provided FREE of charge for all users. This design software is accessible from any internet browser and contains express and custom features to suit both novice and expert users in qPCR. Take the guess work out of probe and primer design - let RealTimeDesign select the optimal sequences for you!
Quenching Mechanisms in Probes
The efficiency of fluorescence quenching is very distance dependent – if the reporter fluorophore and quencher are far apart, there is fluorescence; if the reporter and quencher are close together in space, fluorescence is suppressed. Continue reading to learn more about static and FRET quenching.
Genotyping - qPCR
Real-time PCR methods using fluorescent probes are ideal for low to medium throughput disease-association studies. Review the probe types that Biosearch Technologies offers for this application, and learn how to design assays for genotyping.
Multiplexing qPCR
Multiplexed qPCR combines several PCR assays together into one but resolves the reactions using reporter dyes with distinct spectra. Only Biosearch offers fluorophores and quenchers that span the spectrum to maximize your multiplexing for 2-plex, 3-plex, 4-plex, and 5-plex qPCR.
Gene Expression - qPCR
Learn how Biosearch Technologies can aide your Gene Expression application.
DNA Synthesis
In addition to being a custom oligonucleotide manufacturer, Biosearch Technologies is a major supplier of DNA synthesis reagents to oligo synthesis companies around the world. Not familiar with the mechanics behind solid-phase synthesis? Here is a brief overview.
Dyes from Biosearch Technologies
As inventors of the Black Hole Quencher® (BHQ®) dyes, we design our dyes with the goal of providing solutions for you, the scientist. Our vibrant fluorophores and versatile quenchers are valuable tools for many technologies including: Real-time qPCR, Multiplex qPCR, SNP Genotyping, and FRET assays. See below to learn more about our dyes and the technologies they support.
Stellaris FISH Probes are used for single molecule RNA detection by fluorescence in situ hybridization (FISH). The Stellaris FISH method is a much improved and simplified FISH technology that requires no probe preparation and can be performed in four easy steps.
RealTimeDesign Software
RealTimeDesign™ is our web-based qPCR assay design software provided FREE of charge for all users. This design software is accessible from any internet browser and contains express and custom features to suit both novice and expert users in qPCR. Take the guess work out of probe and primer design - let RealTimeDesign select the optimal sequences for you!
Quenching Mechanisms in Probes
The efficiency of fluorescence quenching is very distance dependent – if the reporter fluorophore and quencher are far apart, there is fluorescence; if the reporter and quencher are close together in space, fluorescence is suppressed. Continue reading to learn more about static and FRET quenching.
Genotyping - qPCR
Real-time PCR methods using fluorescent probes are ideal for low to medium throughput disease-association studies. Review the probe types that Biosearch Technologies offers for this application, and learn how to design assays for genotyping.
Multiplexing qPCR
Multiplexed qPCR combines several PCR assays together into one but resolves the reactions using reporter dyes with distinct spectra. Only Biosearch offers fluorophores and quenchers that span the spectrum to maximize your multiplexing for 2-plex, 3-plex, 4-plex, and 5-plex qPCR.
Gene Expression - qPCR
Learn how Biosearch Technologies can aide your Gene Expression application.
DNA Synthesis
In addition to being a custom oligonucleotide manufacturer, Biosearch Technologies is a major supplier of DNA synthesis reagents to oligo synthesis companies around the world. Not familiar with the mechanics behind solid-phase synthesis? Here is a brief overview.
Dyes from Biosearch Technologies
As inventors of the Black Hole Quencher® (BHQ®) dyes, we design our dyes with the goal of providing solutions for you, the scientist. Our vibrant fluorophores and versatile quenchers are valuable tools for many technologies including: Real-time qPCR, Multiplex qPCR, SNP Genotyping, and FRET assays. See below to learn more about our dyes and the technologies they support.
