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2009 H1N1 ValuPanel Reagents

Influenza A Subtyping ValuPanel Reagents

Fluorogenic Probes and Primers

Dual-Labeled BHQ® Probes

BHQplus™ Probes

Molecular Beacons

Scorpions® Primers

Amplifluor® Primers

Plexor™ Primers

Calibration and Reference Dyes

SuperROX™ Reference Dye

Custom Oligonucleotides

Build Your Custom Oligo

Purification Options

Modifications

Fluorophore Labels

Quencher Labels

Biotin, Amine, Phosphate, Thiol, Spacer Modifications

Other Specialty Modifications

Oligos in Plates

Luminex® Oligos

DNA/RNA Synthesis Reagents

Black Hole Quencher® Reagents

Black Hole Quencher® Amidites

Black Hole Quencher® Columns & CPGs

Other Quencher Reagents

Dabsyl Amidites

Dabcyl Columns & CPGs

Proprietary Fluorophore Reagents

CAL Fluor Dye Amidites

CAL Fluor Dye Columns & CPGs

Quasar Dye Amidites

Quasar Dye Columns & CPGs

Pulsar Dye Columns & CPGs

Generic Fluorophore Reagents

Fluorescein Amidites

Fluorescein Columns & CPGs

TAMRA Amidites

TAMRA Columns & CPGs

TET Amidites

HEX Amidites

ROX Columns & CPGs

DNA Modification Reagents

Amino Modifying Amidites

Amino Modifying Columns & CPGs

Biotinylating Amidites

Biotinylating Columns & CPGs

Phosphorylating Amidites

Phosphorylating Columns & CPGs

Thiol Modifier Amidites

Thiol Modifier CPGs

Spacer Modifier Amidites

Spacer Modifier Columns & CPGs

Standard DNA Synthesis Reagents

deoxyAdenosine d(A) Columns and CPGs

deoxyCytidine d(C) Columns and CPGs

deoxyGuanosine d(G) Columns and CPGs

Thymidine and deoxyUridine d(U) Columns and CPGs

deoxyInosine & deoxyUridine Amidites

deoxyInosine Columns and CPGs

Universal Support Columns and CPGs

Mixed Base Columns and CPGs

Aminopropyl CPGs

Standard RNA Synthesis Reagents

Adenosine Columns and CPGs

Cytidine Columns and CPGs

Guanosine Columns and CPGs

Uridine Columns and CPGs

DNA Purification Products

MicroSync II, Solvent Resistant Vacuum Manifold System

MicroPure II Columns for Oligo Purification

SuperPure Columns for Oligo Purification

DNA Synthesizer Accessories

Empty Columns and Accessories for Automated DNA Synthesizers

Labeling Reagents

Black Hole Quencher Labeling Reagents

Black Hole Quencher® Carboxylic Acids, Active Esters and Amines

Black Hole Quencher Peptide Resins

Proprietary Fluorophore Labeling Reagents

CAL Fluor® Dye Carboxylic Acids

Quasar® Dye Carboxylic Acids & Active Esters

Generic Fluorophore Labeling Reagents

Fluorescein Isomer Carboxylic Acids

TAMRA Isomer Carboxylic Acids

JOE Active Ester

Non-Fluorescent Modification Labeling Reagents

Biotin Active Esters

Methylene Blue Active Ester

Immunochemicals

Hapten Reporter Groups and Conjugates

Biotin Reagents

DNP (2,4-Dinitrophenyl) Reagents

NIP (4-Hydroxy-3-iodo-5-nitrophenylacetyl) Reagents

NP (4-Hydroxy-3-nitrophenylacetyl) Reagents

PC (Phosphoryl Choline) Reagents

TNP (2,4,6-Trinitrophenyl) Reagents

Protein Conjugates

ABA (4-Azobenzenearsonate)-Protein Conjugates

DNP (2,4-Dinitrophenyl)-Protein Conjugates

NIP (4-Hydroxy-3-iodo-5-nitrophenylacetyl)-Protein Conjugates

NP (4-Hydroxy-3-nitrophenylacetyl)-Protein Conjugates

PC (Phosphoryl Choline)-Protein Conjugates

TNP (2,4,6-Trinitrophenyl)-Protein Conjugates

Fluorophore-Protein Conjugates

Other Protein Conjugates

FICOLL Conjugates

DNP (2,4-Dinitrophenyl)-FICOLL Conjugates

NIP (4-Hydroxy-3-iodo-5-nitrophenylacetyl)-FICOLL Conjugates

NP (4-Hydroxy-3-nitrophenylacetyl)-FICOLL Conjugates

TNP (2,4,6-Trinitrophenyl)-FICOLL Conjugates

Fluorophore-FICOLL Conjugates

Fluorophore Conjugates

Fluorescein Conjugates

Cell Biology

N1-Guanyl-1,7-diaminoheptane (GC7)

Promotions

Services

Contract Manufacturing

Oligos for Molecular Diagnostics

Custom Analyte Specific Reagents (ASRs)

Kit and OEM Manufacturing

Support

FAQs

Top Questions

Does Biosearch have a list of all dyes available for DNA labeling?

How do I determine the amount of diluent to add to my probe to prepare a stock concentration?

How many PCR reactions will I be able to run with my probe or primer?

I am a beginner at Real Time PCR, do you have any information to help me design my Dual-labeled assay?

What are the recommended storage conditions for dual-labeled probes?

What is the difference between the FAM-BHQ ValuProbe priced at $95 and the $150 FAM-BHQ probe?

When ordering immunochemical conjugates online, I saw that there is a choice for loading. What does this mean? How do I know which loading ratio to choose?

Where can I find usage information and chemical properties for the products you offer?

Which dyes are compatible with my thermal cycler?

Why does the synthesis scale ordered for my oligonucleotide not correspond with the final yield?

What are "wobbles"?

Why is it that when a sequence contains a ‘wobble’ it has variable functionality?

What formulations of BHQ-1 and BHQ-2 dye do you offer for internal modifications of oligos?

Can Biosearch probes (and dyes) be used for in-situ hybridization (FISH) studies?

DNA Synthesis Reagents

Which synthesis column will work best with my machine?

I am getting low coupling efficiency with amidites, any suggestions?

Custom Oligo Synthesis

Does Biosearch measure A260/A280 ratios of the oligos?

How do I know what fluorophore to pick for my Dual-labeled probe?

I have ordered a 5' thiol C6 modification on my custom oligo, what form does that come in and how do I create a free thiol?

I received my oligonucleotide order, but the vial looks empty. Where is the oligonucelotide?

What are the recommended storage conditions for dual-labeled probes?

What buffer should I resuspend my oligo in?

What different oligonucleotide purification options does Biosearch offer?

What is the proper notation if I want to use a deoxyinosine within my oligo sequence?

What types of purification does Biosearch offer and how do they compare?

Why does the synthesis scale ordered for my oligonucleotide not correspond with the final yield?

Why is BHQ-3 not an option when selecting Quasar or Pulsar dye labels?

Why were my oligonucleotides shipped at room temperature?

Does Biosearch have a list of all available DNA modifications?

Is there a limit to the length of oligos Biosearch will synthesize?

Are unlabeled primers modified with a 3' phosphate?

Does my oligonucleotide have a phosphate on the 5’ or 3’ end?

Do you make primers that are unextendable?

Does Biosearch synthesize Minor Groove Binder DNA probes?

Are Locked Nucleic Acids (LNA) available through Biosearch?

What is the difference between a BHQ and BHQplus Probe?

What is the difference between static quenching and FRET?

Can I use the Nanodrop to measure the concentration of synthetic oligos?

qPCR Assay Design & Optimization

Do I need to calibrate my instrument to detect your Pulsar® 650 dye?

How are fluorescent labels on dual-labeled probes accounted for when measuring the absorbance?

How do freeze thaw cycles affect oligonucleotides?

How do I calibrate my instrument for the CAL Fluor Dyes?

How do I determine the amount of diluent to add to my probe to prepare a stock concentration?

How do I determine which design mode to use in RealTimeDesign?

How do I enter in a SNP, MNP or InDel sequence into RealTimeDesign?

How do I find the primers and probe sequences after I design them?

How do I know what fluorophore to pick for my Dual-labeled probe?

How do I order primers and probes after I design them?

How do I quantitate oligonucleotides by spectrophotometer?

How many PCR reactions will I be able to run with my probe or primer?

I am having a problem with background drift with my Dual-labeled probe, what could be happening?

I am not getting any signal generation with my Dual-labeled probe. What could be going on here?

I am performing Real Time PCR and for my negative controls I am getting products. What could be going on here?

I am using a BHQ labeled probe, how do I adjust my thermal cycler’s settings to account for the quencher?

If I have my own primer designs, can RealTimeDesign (RTD) design only the probe for me?

If I left my oligos sitting on the lab bench over the weekend, are they still ok to use?

If I order a dual-labeled probe with an internal BHQ modification, what will be at the 3’ end of the probe?

Is there any way of designating a specific region on my sequence where RealTimeDesign can design my primers, probes and assays?

What are Tandem Repeats and Mask Tandems in RealTimeDesign?

What does the Overall Rank score in RealTimeDesign represent?

What is the difference between the FAM-BHQ ValuProbe priced at $95 and the $150 FAM-BHQ probe?

What is the Failure Count Data in RealTimeDesign?

What is your recommended method for reconstituting and storing oligos?

When I received my Molecular Beacon probe I also received a complement, what is this for?

Where can I find information that explains the differences between each of the probes you offer?

Why is my BHQ-Pulsar probe not detected in a singleplex reaction on the Lightcycler?

I am a beginner at Real Time PCR, do you have any information to help me design my Dual-labeled assay?

Does Biosearch have software available for designing probes and primers?

What are the differences between the parameter settings in your primer and probe design software (RealTimeDesign)?

How is the Tm calculated in Biosearch’s Real Time Design Software?

Is there a formula for calculating the efficiencies of singleplex PCR reactions?

Dyes

How do I calibrate my instrument for the CAL Fluor Dyes?

How do I know what fluorophore to pick for my Dual-labeled probe?

Why is BHQ-3 not an option when selecting Quasar or Pulsar dye labels?

Does Biosearch have a list of all dyes available for DNA labeling?

Does Biosearch Technologies offer NED fluorophores?

Which dyes are compatible with my thermal cycler?

What is the difference between Black Hole Quenchers (BHQ) and TAMRA?

What is the difference between Biosearch’s Quasar® dyes and the Cy™ dyes?

How do you determine the brightness of a dye?

What are the quantum yields of the Black Hole Quenchers?

Are Biosearch dyes compatible with DNA sequencing or fragment analyzers?

Protein & Peptide Labeling

Which one of your Black Hole Quenchers do you recommend using to label proteins?

How stable are the CAL Fluor and BHQ dyes when conjugated to peptides in solution?

Immunochemicals

I am planning an ELISA using DNP-BSA to measure the DNP-specific antibody titer in serum. Do you have any information on which conjugation ratio (3 or 31) I should use for this assay?

When ordering immunochemical conjugates online, I saw that there is a choice for loading. What does this mean? How do I know which loading ratio to choose?

Are Biosearch’s immunochemical products alum precipitated?

Is it possible to order NP-LPS and NP-BSA with the same NP hapten loading?

Product Literature

Where can I find usage information and chemical properties for the products you offer?

Does Biosearch have Material Safety Data Sheets (MSDS) available?

Ordering & Sales Information

How do I order primers and probes after I design them?

How should orders be placed for shipment to locations outside the United States?

Applications

RealTimeDesign Software

Genotyping - qPCR

Multiplexing qPCR

Gene Expression - qPCR

DNA Synthesis

Dyes from Biosearch Technologies

Black Hole Quencher® Dyes

CAL Fluor® Dyes

Quasar® Dyes

Pulsar® 650 Dyes

SuperRox™ Dye

Dye Calibration Standards

Videos

Making of the Probes and Primers for CDC's Swine Flu Test Kit - Inside Biosearch Technologies Video

Real-time PCR Probe Animation Video

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