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Black Hole Quencher Labeling Reagents #

Biosearch’s proprietary BHQ dyes were designed to permit efficient quenching across the visible spectrum from 480 nm into the near IR, making it possible to utilize reporter dyes that emit anywhere within this range.

Black Hole Quencher Absorption Curve
Normalized absorption spectra of the BHQ dyes illustrate their range of quenching through FRET
BHQ dyes work through a combination of FRET and static quenching to enable researchers to avoid the residual background signal common to fluorescing quenchers such as TAMRA, or low signal : noise ratio as with dabcyl.

BHQ quenchers have broad absorption spectra and can be paired with reporter dyes that emit in the following ranges:

BHQ-0: 430-520 nm
BHQ-1: 480-580 nm
BHQ-2: 560-670 nm
BHQ-3: 620-730 nm

Learn more about the Black Hole Quencher dye technology.
Black Hole Quencher® Carboxylic Acids, Active Esters and Amines
Biosearch offers Black Hole Quencher carboxylic acids and amines for coupling to amine or carboxy groups.


Black Hole Quencher Peptide Resins
Resins for the synthesis of BHQ-terminal peptides.