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| DNA Purification Products |
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MicroSync II, Solvent Resistant Vacuum Manifold System
A universal microplate-based vacuum manifold for purification column processing.
MicroPure II Columns for Oligo Purification
Biosearch’s MicroPure II columns (MP-1602) are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a 5 mL syringe barrel packed with 200 mg of high performance hydrophobic polymeric resin. The method relies on strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. At least 1 µmol or 50 O.D's of crude DNA can be applied to the column. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
SuperPure Columns for Oligo Purification
Biosearch’s SuperPure columns are intended for reverse-phase, low pressure purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene) thereby allowing separation from truncated sequences which, due to a lack of DMT group, do not bind.
A universal microplate-based vacuum manifold for purification column processing.
MicroPure II Columns for Oligo Purification
Biosearch’s MicroPure II columns (MP-1602) are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a 5 mL syringe barrel packed with 200 mg of high performance hydrophobic polymeric resin. The method relies on strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. At least 1 µmol or 50 O.D's of crude DNA can be applied to the column. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
SuperPure Columns for Oligo Purification
Biosearch’s SuperPure columns are intended for reverse-phase, low pressure purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene) thereby allowing separation from truncated sequences which, due to a lack of DMT group, do not bind.
